Scholarly articles for prolyl hydroxylase activity assay kit

Assay Solution FKBP4 Antibody

FKBP4 is part of the immunophilin protein family which takes part in immunoregulation and necessary cellular processes concerning protein folding and trafficking FKBP4 is a cis-trans prolyl isomerase that connects to the immunosuppressants FK506 and rapamycin FKBP4 has high structural and functional similarity to FKBP1A though FKBP4 does not have immunosuppressant activity when complexed

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RESEARCH Open Access Construction of a recombinant

Prolyl hydroxylase domain 3 (PHD3) is a hypoxia inducible factor-α (HIFα) regulator it degrades HIFα in the presence of oxygen Recently there have been an increasing number of studies about the role of PHD3 in proliferation and apoptosis of cancer cells However most of the evidence for the role of PHD3 is observational and little is known of the molecular mechanism In our current

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Prolyl hydroxylase inhibitors increase the production of

Read Prolyl hydroxylase inhibitors increase the production of vascular endothelial growth factor by periodontal fibroblasts Journal of Periodontal Research on DeepDyve the largest online rental service for scholarly research with thousands of academic publications available at your fingertips

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Studies on spiro[4 5]decanone prolyl hydroxylase domain

Prolyl-4-hydroxylation of prolyl residue in hypoxia inducible factor α (HIF-α) subunits signals for degradation via the ubiquitin proteasome system 2OG 2-oxoglutarate PHD1-3 human prolyl hydroxylase enzymes 1–3 VHL-E3 ligase: the von Hippel Lindau protein (VHL) is the targeting component of a ubiquitin E3 ligase system B Examples of PHD inhibitors in clinical trials Roxadustat

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Iron deficiency decreases renal 25

Also renal 25-hydroxyvitamin D 3-1α-hydroxylase (1α-hydroxylase) converts 25-hydroxyvitamin D to 1 25-dihydroxyvitamin D the active form of vitamin D and contains iron as a cofactor Therefore as a consequence of iron deficiency these iron-containing enzymes activity may be lowered and hence metabolic disorder of collagen and vitamin D may occur In previous studies researchers

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hypoxic regulation of neutrophilic inflammation Prolyl

Prolyl hydroxylase 3 (PHD3) is essential for hypoxic regulation of neutrophilic inflammation in humans and mice Sarah R Walmsley 1 Edwin R Chilvers 2 Alfred A Thompson 1 Kathryn Vaughan 1 Helen M Marriott 1 Lisa C Parker 1 Gary Shaw Selina Parmar Martin Schneider 3 Ian Sabroe 1 David H Dockrell 4 Marta Milo 5 Cormac T Taylor 6 Randall S Johnson 7 Christopher W Pugh 8 Peter J

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Role of prolyl hydroxylase domain proteins in the

Since prolyl hydroxylase domain proteins (PHDs) can metabolize cytosolic aKG we sought to explore the role of this enzyme in the regulation of b-cell function The oxygen-sensing PHDs regulate the stability of hypoxia-inducible factor 1a (HIF1a) as well as other proline-containing proteins by catalyzing the hydrox-ylation of proline residues This reaction is dependent on sufficient levels

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Biochemical Characterization of the Prolyl 3

Prolyl 3-hydroxylase 1 modifies a single proline residue in the α chains of type I II and III collagens to (3S)-hydroxyproline The peptidyl-prolyl cis-trans isomerase activity of cyclophilin B was shown previously to catalyze the rate of triple helix formation Here we show that cyclophilin B in the complex shows peptidyl-prolyl cis-trans isomerase activity and that the P3H1CRTAPCypB

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Activation of the HIF Prolyl Hydroxylase by the Iron

02 11 2011The loss of prolyl hydroxylase activity was traced to a decrease in iron loading of the enzyme which could be restored with recombinant PCBP1 PCBP1 physically interacted with PHD2 indicating that PCBP1 likely acts as an iron chaperone for PHD2 Our studies also suggest a direct role for PCBPs in the activation of the asparaginyl hydroxylase FIH1 RESULTS Accumulation of

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A nonradioactive 96

Prolyl hydroxylase activity can be determined by measuring the hydroxylation-coupled consumption of 2-oxoglutarate An assay based on the measurement of the amount of 14 CO 2 released during decarboxylation of 2-oxo[1-14 C]glutarate has been described recently This assay was used to characterize prolyl hydroxylase activities in the lysates

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Hypoxia Up

03 10 2003Hypoxia reduces activity of prolyl hydroxylases (PHD) that hydroxylate specific proline residues in the oxygen-dependent degradation domain (ODD) of hypoxia-inducible factor-1α (HIF-1α) As a consequence HIF-1α accumulates and promotes hypoxic tolerance by activating gene transcription This paper identifies the three forms of PHDs in rats and shows that a period of hypoxia selectively

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Pyruvate Kinase M2 Is a PHD3

The pyruvate kinase isoforms PKM1 and PKM2 are alternatively spliced products of the PKM2 gene PKM2 but not PKM1 alters glucose metabolism in cancer cells and contributes to tumorigenesis by mechanisms that are not explained by its known biochemical activity We show that PKM2 gene transcription is activated by hypoxia-inducible factor 1 (HIF-1) PKM2 interacts directly with the HIF-1α

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A rapid assay for prolyl 3

Assay of Prolyl 3-Hydroxylase Activity The prolyl 3-hydroxylase reaction under standard conditions was carried out for 40 min at 20C in a final volume of 2 0 ml containing 1 000 000 dpm 2 3-T-proline-labeled substrate 0 08 mm FeS04 2 mm ascorbate 0 5 mm 2-oxoglutarate 0 2 mg/ml of catalase 2 mg/ml of bovine serum albumin 0 1 mm dithiothreitol and 0 05 m Tris-HCI (pH 7 8 at 25C) (2

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Direct and continuous assay for prolyl 4

15 03 2009Prolyl 4-hydroxylase (P4H) is a non-heme iron dioxygenase that catalyzes the post-translational hydroxylation of (2S)-proline (Pro) residues in protocollagen strands The resulting (2S 4R)-4-hydroxyproline (Hyp) residues are essential for the folding secretion and stability of the collagen triple helix P4H uses α-ketoglutarate and O 2 as co-substrates and forms succinate and CO 2 as well as

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Fluidigm

Inhibition of Prolyl Hydroxylase Protects against 1-Methyl-4-phenyl-1 2 3 6-tetrahydropyridine-induced Neurotoxicity Lee D W Rajagopalan S Siddiq A et al Hypoxia-inducible factor (HIF) plays an important role in cell survival by regulating iron antioxidant defense and mitochondrial function Pharmacological inhibitors of the iron

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Procollagen

Procollagen-proline dioxygenase commonly known as prolyl hydroxylase is a member of the class of enzymes known as alpha-ketoglutarate-dependent hydroxylases These enzymes catalyze the incorporation of oxygen into organic substrates through a mechanism that requires alpha-Ketoglutaric acid Fe 2+ and ascorbate This particular enzyme catalyzes the formation of (2S 4R)-4

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Hydroxyproline Assay Kit (Perchlorate

Hydroxyproline Assay Kit (Perchlorate-Free) Catalog Number STA-675 96 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures 2 Hydroxyproline is an amino acid that is synthesized from the irreversible post-translational hydroxylation of proline by prolyl hydroxylase Hydroxyproline is found almost exclusively in the protein collagen in the Y position of the repeating

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Ethyl 3 4

hydroxybenzoate (EDHB) a prolyl hydroxylase enzyme inhibitor in modulating adaptive responses to hypobaric hypoxia (HH) in rat brain Male Sprague–Dawley rats treated with EDHB (75 mg/kg for 3 days) were subjected to acute HH exposure at 9144 m (30 000 ft) for 5 h Ani-mals were assessed for transvascular leakage and edema formation in brain and role of key inflammatory markers along

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Molecular mechanisms for the activity of PX

Several other agents have been reported to lower HIF-1α protein levels including topoisomerase I and II inhibitors (38 39) phosphatidylinositol 3-kinase inhibitors heat shock protein 90 inhibitors microtubule cytoskeleton disrupting agents HIV protease inhibitors and YC-1 an activator of soluble guanyl cyclase that stimulates prolyl hydroxylase activity None of these agents is

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Research Paper Prolyl hydroxylase domain protein 3 and

stabilization and activity are regulated by prolyl hydroxylase domain (PHD)-1 -2 -3 and factor inhibiting HIF (FIH) This study investigated the relation between these oxygen sensors and the clinical behaviors and prognosis of hepatocellular carcinoma (HCC) Tissue microarray and RT-PCR analysis of tumor tissues and adjacent non-tumor liver tissues revealed that mRNA and protein levels of

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Prolyl

Die Prolyl-4-Hydroxylase (4-PH) (auch: Procollagenprolin-Dioxygenase EC 1 14 11 2) ist ein Enzymkomplex in Eukaryoten (Eucaryota) der in Gewebetieren oder Wirbeltieren die Hydroxylierung von Prolylresten in Proteinen katalysiert Dabei handelt es sich um eine posttranslationale Modifikation Sie ist essentiell fr die Biosynthese des Kollagens

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Research Paper Prolyl hydroxylase domain protein 3 and

stabilization and activity are regulated by prolyl hydroxylase domain (PHD)-1 -2 -3 and factor inhibiting HIF (FIH) This study investigated the relation between these oxygen sensors and the clinical behaviors and prognosis of hepatocellular carcinoma (HCC) Tissue microarray and RT-PCR analysis of tumor tissues and adjacent non-tumor liver tissues revealed that mRNA and protein levels of

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Prolyl 3

28 05 2004We show this enzyme to have prolyl 3-hydroxylase activity in a previously described assay with full-length procollagen Gelatin-Sepharose affinity chromatography used previously to identify proteins that bind to denatured collagen ( 23 24 ) was used here to demonstrate the ability of P3H1 to specifically bind to denatured collagen as well as to interact with other rER proteins as a

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