Modern Liquid Chromatography of Macromolecules

Desalting and Gel Filtration Chromatography

Desalting and buffer exchange use gel filtration chromatography to separate soluble macromolecules from smaller molecules Desalting and buffer exchange are two of the most widely used gel filtration chromatography applications and both can be performed using the same materials

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Molecular topological fractionation of macromolecules

26-8-2008A process for characterizing a sample comprising a population of linear macromolecules of interest (104) and a population of long chain branched macromolecules of interest (103) the process including four steps The first step is to provide a flow through separating medium (100) and a liquid eluant (101) in which the macromolecules of interest dissolve the separating medium defining flow

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Chromatographic Links

Chromatography Techniques Journal of Liquid Chromatography Related Technologies Mass Spectrometry and Chromatography Publications Chemical Analysis Peak Magazine at Agilent MAC Mod: This HPLC Column Companion - Introduction and Table Contents Journal of Chromatographic Science from 1996 to the current 1998 issue Journal of Analytical Toxicology

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GPC/SEC/GFC

GPC (Gel Permeation Chromatography) GFC (Gel Filtration Chromatography) and SEC (Size Exclusion Chromatography) are names used to describe the same liquid column chromatographic technique required to determine important properties of macromolecules also known as polymers biopolymers polysaccharides or proteins

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Methods of Protein Purification: 4 Methods

High performance liquid chromatography or high pressure liquid chromatography is a form of chromatography applying high pressure to drive the solutes through the column faster This means that the diffusion is limited and the resolution is improved The most common form is "reversed phase" HPLC where the column material is hydrophobic

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Chromatography equipment available at Polymer Science

GPC / SEC Gel permeation chromatography/size exclusion chromatography (GPC/SEC) is the technique of choice for rapid and reliable characterization of macromolecules on the basis of molar mass (Mn) molar mass distribution (MMD) and dispersity (Đ) As the most commonly applied LC technique the separation is based on the hydrodynamic volume ("effective" molecular size) of

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Liquid chromatography packing materials

Liquid chromatography using a reverse phase packing has been found to be an effective tool in both qualitative and quantitative analysis for drug substances in blood serum or plasma Typically the reverse phase packing material is made up of bonded alkyl silica and most typically the packing is a porous silica having octadecylsilane (ODS) bonded to it

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Desalting and Gel Filtration Chromatography

Desalting and buffer exchange use gel filtration chromatography (or size-exclusion chromatography) to separate soluble macromolecules from smaller molecules When an aqueous solution is used to transport the sample through the column the technique is referred to as gel-filtration chromatography

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Liquid chromatography under limiting conditions of

Liquid chromatography of polymers under limiting conditions (LC-LC) of enthalpic interactions employs different velocities of small (eluent or auxiliary liquid) molecules and large (sample) molecules within a HPLC column Slowly moving small molecules of a liquid act as a "barrier" hindering fast progression of large molecules eluting with exclusion retention mechanism

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Analysis of trace pharmaceuticals and related compounds

Liquid Chromatography LC-MS/MS has been widely used because of its high sensitivity and selectivity It is suitable for the determination of a wide range of polar and amphiphilic compounds Because pharmaceuticals are often polar and non-volatile they can be analyzed by LC without derivatization

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Silica Hybrid Silica Hydride Silica and Non

Introduction The analysis of basic compounds by liquid chromatography (LC) continues to be of interest as over 70% of pharmaceuticals are bases () while peptides and proteins which have several basic and acid functions are an emerging class of therapeutic agents currently being developed by many pharmaceutical companies (2– 4) Thus all the discussions are devoted to ionizable compounds

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OSA

A novel surface plasmon resonance (SPR) thermometer based on liquid crystal (LC) filled hollow fiber is demonstrated in this paper A hollow fiber was internally coated with silver and then filled with LC The SPR response to temperature was studied using modeling and verified experimentally The results demonstrated that the refractive index of LC decreases with the increasing temperature and

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Chromatography Library Modern Liquid Chromatography of

Chromatography Library Modern Liquid Chromatography of Macromolecules() PDF JOURNAL OF CHROMATOGRAPHY LIBRARY - volume 25 modern liquid chromatography of macromolecules This Page Intentionally Left Blank JOURNAL OF CHROMATOGRAPHY LIBRARY - volume 25 modern liquid chromatography of macromolecules B G Belenkii and L Z Vilen

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Cocos: Worldwide

The carbohydrates (polysaccharides oligosaccharides and monosaccharides) in three parts (epidermis middle and inner part) of P cocos were qualitatively and quantitatively characterized by high performance gel permeation chromatography coupled with charged aerosol detector (HPGPC-CAD) and ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC

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Fundamental Properties of Packing Materials for Liquid

Reversed-phase liquid chromatography is the inverse of normal-phase liquid chromatography However it seems that HILIC is not the same as normal-phase liquid chromatography If hydrophobic interaction is eliminated from the retention mechanisms in liquid chromatography the remaining interactions are hydrogen bonding and Coulombic interaction

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Chromatographic Links

Chromatography Techniques Journal of Liquid Chromatography Related Technologies Mass Spectrometry and Chromatography Publications Chemical Analysis Peak Magazine at Agilent MAC Mod: This HPLC Column Companion - Introduction and Table Contents Journal of Chromatographic Science from 1996 to the current 1998 issue Journal of Analytical Toxicology

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Hplc Of Macromolecules

Author by : R W A Oliver Language : en Publisher by : Oxford University Press on Demand Format Available : PDF ePub Mobi Total Read : 96 Total Download : 839 File Size : 52 7 Description : HPLC stands for high pressure (or performance) liquid chromatography and is a standard biochemical technique for separating molecules This volume covers the larger biomolecules - oligosaccharides

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The Ins and Outs of Chromatography

What is chromatography? Chromatography is a scientific process in separation science the part of chemistry concerned with the separation of compounds and mixtures Generally speaking the two significant categories are preparative and analytical Analytical chromatography uses smaller sample sizes and the primary objective of separating compounds is to identify their components A good

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Precipitation

Free Online Library: Precipitation-redissolution liquid chromatography of styrene-ethyl acrylate copolymers by Polymer Engineering and Science Engineering and manufacturing Science and technology general Block copolymers Research Fractional distillation Methods Fractionation Liquid chromatography Usage

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Basic Principles of Chromatography

A much larger number of tubes were required to separate mixtures of substances with close partition coefficients which made this type of countercurrent extraction very tedious Modern liquid-liquid partition chromatography (Sect 12 4 2) is much more efficient and convenient

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Love

Chromatography began to take its modern form following the work of Archer John Porter Martin and Richard Laurence Millington Synge in the 1940s and 1950s Liquid chromatography Liquid chromatography Columns used with an FPLC can separate macromolecules based on size charge distribution (ion exchange) hydrophobicity

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